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Histopathologic and serological landscapes of thyroid between the young and age-related subclinical hypothyroidism (AR-SCH) mice. (A, B) Hematoxylin-eosin (H&E) images of thyroid tissue sections derived from young (A) and AR-SCH mice (B) (Scale bars = 50 μm). (C-F) Quantitative analysis of <t>the</t> <t>anti-thyroglobulin</t> antibody (TGAB) (C), T4 (D), T3 (E), and serum thyrotropin <t>(TSH)</t> (F) concentrations between the young and AR-SCH thyroid. * P -value < 0.05, ** P -value < 0.01. Data are presented as mean ± standard deviation (SD), n = 6. (G, H) Immunofluorescence micrographs of thyroid peroxidase (TPO) expression (red) counterstained with 4’,6-diamidino-2-phenylindole (DAPI, blue) in young (G) and AR-SCH (H) mouse thyroid tissues (Scale bars = 50 μm).
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Histopathologic and serological landscapes of thyroid between the young and age-related subclinical hypothyroidism (AR-SCH) mice. (A, B) Hematoxylin-eosin (H&E) images of thyroid tissue sections derived from young (A) and AR-SCH mice (B) (Scale bars = 50 μm). (C-F) Quantitative analysis of the anti-thyroglobulin antibody (TGAB) (C), T4 (D), T3 (E), and serum thyrotropin (TSH) (F) concentrations between the young and AR-SCH thyroid. * P -value < 0.05, ** P -value < 0.01. Data are presented as mean ± standard deviation (SD), n = 6. (G, H) Immunofluorescence micrographs of thyroid peroxidase (TPO) expression (red) counterstained with 4’,6-diamidino-2-phenylindole (DAPI, blue) in young (G) and AR-SCH (H) mouse thyroid tissues (Scale bars = 50 μm).

Journal: American Journal of Translational Research

Article Title: Integrated multi-omics atlas reveals PTM-driven signaling and immunologic remodeling in a mouse model of age-related subclinical hypothyroidism

doi: 10.62347/PQYE9899

Figure Lengend Snippet: Histopathologic and serological landscapes of thyroid between the young and age-related subclinical hypothyroidism (AR-SCH) mice. (A, B) Hematoxylin-eosin (H&E) images of thyroid tissue sections derived from young (A) and AR-SCH mice (B) (Scale bars = 50 μm). (C-F) Quantitative analysis of the anti-thyroglobulin antibody (TGAB) (C), T4 (D), T3 (E), and serum thyrotropin (TSH) (F) concentrations between the young and AR-SCH thyroid. * P -value < 0.05, ** P -value < 0.01. Data are presented as mean ± standard deviation (SD), n = 6. (G, H) Immunofluorescence micrographs of thyroid peroxidase (TPO) expression (red) counterstained with 4’,6-diamidino-2-phenylindole (DAPI, blue) in young (G) and AR-SCH (H) mouse thyroid tissues (Scale bars = 50 μm).

Article Snippet: Finally FT4 (ASB, EK6868), FT3 (KeyGEN, KGC1066), TSH (CUSABIO, CSB-E05116m), and anti-thyroglobulin antibody (TGAB) (CUSABIO CSB-E09543m) were measured by ELISA method on a full-wavelength microplate reader (Thermo Scientific, Multiskan GO).

Techniques: Tissue, Derivative Assay, Analysis, Standard Deviation, Immunofluorescence, Expressing